Upscaling of alkaline pea protein extraction from dry milled and pre-treated peas from laboratory to pilot scale: Optimization of process parameters for higher protein yields

Abstract The upscaling of pea protein extraction from laboratory scale with a centrifuge to pilot decanter was investigated, and the efficiency dry milled pre-treated peas compared. Upscaling is possible since starch under limit detection (< 0.5%). banding pattern sodium-dodecyl-sulfate polyacrylamide gel electrophoresis confirmed that albumins globulins were extracted by alkali extraction. Protein yield increased 59.5% 67.1% for due constant quick discharge matter in centrifuge. For peas, 60.3% 94.3%, which explained an improved cutting separation compared scale. impact acceleration, mass flow, differential speed their respective interactions decanting process determined design experiments. only flow exceeded significance level. However, 5 kg h −1 , acceleration 1000 g $$\times$$ × 50 min led highest 75.6%. obtained yields range 83 96% therefore did not show significant differences yield.